| SH2_Molecular_Cell.pdf | (1.2MB) | Liu, B.A., Jablonowski, K., Raina, M., Arcé, M., Pawson, T., and Nash, P.D. (2006) The Human and Mouse Complement of SH2 Domain Proteins - establishing the boundaries of phosphotyrosine signaling. Molecular Cell 22: 851-868. The paper that this site was created to support as an ongoing project to catalog and understand SH2 domains and SH2 protein. Here, we report a comprehensive bioinformatics, structural, and functional view of the human and mouse complement of SH2 domain proteins. This delimits the set of SH2-containing effectors available for PTK signaling and serves as a general overview of the state of SH2 knowledge. |
| SH2_MolCell_Suppl.pdf | (1.9MB) | Supplementary data for Liu, B.A., Jablonowski, K., Raina, M., Arcé, M., Pawson, T., and Nash, P.D. (2006) The Human and Mouse Complement of SH2 Domain Proteins - establishing the boundaries of phosphotyrosine signaling. Molecular Cell 22: 851-868. |
| SH2_Dendrogram.pdf | (320KB) | Dendrogram of the human complement of SH2 domains. The dendrogram, modified for ease of viewing, shows the sequence similarity between the SH2 domains indicated in the alignment. The initial branching pattern was built from a neighbor-joining tree derived from a clustalW protein sequence alignment of the domains. This was modified by reference to other alignment and tree-building methods (hmmalign and parsimony trees) and by pairwise alignment of SH2 domains. The curved layout was created manually. The overall branching pattern is more informative than that produced by any single automated method, though many branch lengths are semiquantitative. Branches of the tree are colored according to the presumed function of the protein in which each SH2 domain is embedded according to the legend provided. The protein name is indicated in red if one or more structures exist as PDB files (indicated in the SH2 structures table). |
| SH2_Phylogram.pdf | (32KB) | The SH2 domains from human are arranged in a phylogenetic tree (including bootstrap values), and organized according to protein family. |
| SH2_Alignment.pdf | (996KB) | All 120 verified SH2 domains from Human were aligned using ClustalW. The alignment was refined to preserve alignment of known structural elements. The secondary structure is indicated at the top of the alignment as alpha-helix and beta-strands. |
| SH2_GenomicTable.pdf | (96KB) | The 120 SH2 domains contained in 110 proteins identified in the Human and Mouse genome are listed, along with the genomic location of the gene encodding the protein, a list of protein or gene alternate names (aliases), and the Entrez Gene ID for each. |
| SH2_KOMice.pdf | (196KB) | Targeted gene disruptions have been completed for a large subset of SH2 proteins, yielding a wide range of phenotypes. In some cases, compensatory mechanisms have required the disruption of multiple members of a family in order to expose a phenotype. |
| SH2_StructureTable.pdf | (52KB) | The structures of a large number of SH2 domains have been determined, often in complex with peptide or other ligands, and in some cases as part of larger multi-protein complexes. This table provides an overview of the current state of our structural understanding of SH2 domains, with the Protein Database (PDB) structure accession codes for the available structures indicated. |
| SH2_HumanDisease.pdf | (108KB) | Human diseases associated with mutations in SH2-encoding genes. |
| SH2_LigandsTable.pdf | (96KB) | Consensus binding motifs for SH2 domains. The experimentally determined consensus peptide binding motif and associated reference for a sub-set of SH2 domains. |
